Which of you runs an E67 with T42 4L80 and in what vehicle?

It is a 09 GMC Canyon 5.3, 4L80 swapped, with an OS of an express van with 4L80 in the T42 starts in second gear but the signals on the dash are correct. (OS: 24239927). I found help with someone who did it in an 07 TBSS (OS: 24235341), the four shifts works fine, starts on first shift now transmission all fine. But this problem starts when in the dash I haven?t speed signal and TR signal freezed, speed 255km/h and TR signal is 1st gear all time.
*(I don't want to use relays with 4L60 OS)
Tell me about your 4L80 swaps on e67 ecu?

That's an 08 TCM OS just FYI, the Express Vans used it in 08 and I know the TBSS used that OS in 08 (no V8 Colorado in 08 IIRC).

I just grabbed an 09 Express file from the repo, the TCM OS is 24243356. 09 Colorados use the same TCM OS # so a segment swap should be straightforward, wire it as a 09 Express Van 4L80E and you're done.

Bringing this back from the dead, did you ever got your truck working correctly? What did you use after all??

I haven't heard back if the OP got the issue resolved. If you're doing a 80E swap in a stock vehicle, stick with limiting the variables. I.E. if you have an 08 Colorado 5.3 (E67) + 4L60E (T42), keep the matching 08 ECM + TCM OS pair so there won't be issues with CAN comms. I have 4L80E T42 files for both 08 and 09 that (should) work with Silverados, TrailBlazers, and Colorados from the Express Vans. I've found these 4 platforms share the same TCM OS for 08 and 09, so segment swapping from an Express is straightforward.

Yes I just did that, mine is from an 09 and following your advice I got one from an 09 express with the same OS as the tcm I have.